Kinetic–spectrophotometric approach to the ampicillin hydrolytic degradation applied for the histidine determination

The objective of this research was to develop a kinetic-spectrophotometric method for the determination microquantities L-histidine in pure form and dietary supplements. based on kinetics ampicillin degradation by Ni(II) ion as catalyst presence strongly alkaline medium. rate reaction monitored spectrophotometrically measuring increase absorbance at 265 nm function time. same approach used investigation absence histidine. A differential variant tangent process kinetic data. Beer?s law obeyed interval histidine concentration from 1.24 ?g/ml 11.63 with relative standard deviation ranging 8.1% 0.7%. detection limit 0.46 estimated 3S0 criterion. interference effects some metal ions, anions, other molecules were studied assess selectivity. Herein described applied quantification point hypothesis test confirmed that there no significant difference between proposed reference method.